Journal: Journal of Cellular and Molecular Medicine
Article Title: Propofol exposure during late stages of pregnancy impairs learning and memory in rat offspring via the BDNF ‐TrkB signalling pathway
doi: 10.1111/jcmm.12884
Figure Lengend Snippet: Decreased expression levels of BDNF and TrkB in the offspring rats’ hippocampus and the modulatory effects of DHF treatment. Immunohistochemistry staining revealed that the protein levels of BDNF and TrkB in the propofol exposure group (P group) were significant less than those in the control group (C group) ( A – C ). TrkB agonist (7,8‐ DHF ) reversed the expression of BDNF and TrkB. The protein levels of BDNF and TrkB in the 7,8‐ DHF treatment group ( PD group) were higher than those in the P group ( A – C ). Further, 7,8‐ DHF did not change the protein levels of BDNF and TrkB in rat offspring that had not been exposed to propofol ( CD group) ( A – C ). The changes of BDNF and TrkB mRNA expressions were similar to their proteins ( D ). ( A ) BDNF protein immunohistochemistry stain in offspring's hippocampus (400×); ( B ) TrkB protein immunohistochemistry stain in offspring's hippocampus (400×); ( C ) Data represents mean ± S.E. of 20 offspring rats in each group. ( D ) Real time PCR results of BDNF and TrkB mRNA expressions. Data represents mean ± S.E. of 20 offspring rats in each group, * P < 0.05.
Article Snippet: The following main reagents were used in this study: propofol (Diprivan, AstraZeneca UK limited, Italy: jc393, 20 ml: 200 mg); 20% intralipid (2B6061; Baxter, Deerfield, IL, USA); 7,8‐Dihydroxyflavone (50 mg; Abcam, Cambridge, MA, USA); dimethyl sulphoxide (DMSO, 100 ml; Takara, Japan); TRIzol total RNA extraction kit (100 ml; Takara, Ohtsu, Shiga, Japan); RNAstore (100 ml; Takara); diethylpyrocarbonate (DEPC) (100 ml; Takara); MD101‐02 DNA Maker I (2 ml; Takara); Prime ScriptTM RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Code: RR047A for 100 * 20 μl reactions, Takara); SYBR Premix Ex TaqTM II (Tli RNaseH Plus) (Takara Code: RR820A for 200 * 50 μl reactions, Takara); BDNF, TrkB and β‐actin RT‐PCR primers (Sigma‐Aldrich, MA, USA); and DAB Colour Developing Reagent Kit for immunohistochemistry (IHC; DAKO, Carpinteria, CA, USA).
Techniques: Expressing, Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction
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